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Quantitative proteomic analysis of the adipocyte plasma membrane
Matthew J Prior1, Mark Larance, Robert T Lawrence
1Diabetes and Obesity Program, Garvan Institute of Medical Research , Sydney, NSW 2010, Australia.
Journal of Proteome Research
|September 21, 2011
Summary
This study used proteomics to map adipocyte plasma membrane proteins, revealing novel insulin-responsive targets and potential therapeutic avenues for metabolic research and cell biology.
Area of Science:
- Cell Biology
- Metabolic Research
- Proteomics
Background:
- Adipocytes are crucial regulators of mammalian metabolism.
- Understanding adipocyte plasma membrane (PM) protein composition is vital for metabolic research.
Purpose of the Study:
- To define the protein composition of the adipocyte PM.
- To identify novel proteins and insulin-responsive elements in adipocytes.
Main Methods:
- Quantitative proteomics was employed.
- Adipocyte PM proteins were analyzed with and without insulin stimulation.
Main Results:
- A high-confidence list of 486 PM proteins was identified.
- 52 potential novel cell surface proteins were discovered, including adiponectin receptor family members and hydrolases.
- Novel insulin-responsive proteins like sodium/hydrogen exchanger (NHE6) and collagens III/VI were identified.
- Evidence for a functional association between the PM and ER in adipocytes was found.
Conclusions:
- This research provides a comprehensive resource for adipocyte and PM biology.
- Identified proteins represent potential therapeutic targets for modulating adipocyte function and metabolic processes.
