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Direct estimation of serological H-Y antigen by flow cytometry
M Kent1, S Wachtel, H T Thaler
1College of Agricultural and Life Sciences, University of Wisconsin, Madison 53706-1284.
Human Genetics
|June 1, 1990
Abstract:
This study was undertaken to evaluate the efficiency of flow cytometry in the detection of the serological H-Y antigen, and to survey expression of H-Y in the normal human population. Peripheral blood leukocytes (granulocytes) were reacted with monoclonal H-Y antibody, gw-16, and with FITC-conjugated goat anti-mouse IgG, and then were scored for fluorescence in the flow cytometer. Assigned H-Y phenotype was correlated accurately with sex phenotype in 33 out of 38 women and 62 out of 65 men. Data were evaluated by exploratory data analysis (EDA).