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Hyaluronate can function as a cell adhesion molecule and CD44 participates in hyaluronate recognition
K Miyake1, C B Underhill, J Lesley
1Immunobiology & Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City 73104.
The Journal of Experimental Medicine
|July 1, 1990
Summary
Monoclonal antibodies targeting CD44/Pgp-1 block cell adhesion by inhibiting the interaction between hyaluronate and CD44. This finding is crucial for understanding cell recognition in lympho-hemopoiesis.
Area of Science:
- Immunology
- Cell Biology
- Hematopoiesis
Background:
- Monoclonal antibodies (mAbs) were developed targeting CD44/Pgp-1.
- These antibodies inhibited lymphoid and myeloid cell production in bone marrow cultures.
- Hyaluronate was identified as a potential ligand for CD44.
Purpose of the Study:
- To investigate the role of hyaluronate and CD44 in cell adhesion.
- To determine the mechanism by which CD44/Pgp-1 antibodies affect cell interactions.
- To elucidate the cellular location of interacting molecules in adhesion.
Main Methods:
- Cell adhesion assays using hyaluronate-coated surfaces and stromal cell monolayers.
- Inhibition studies using hyaluronidases and anti-CD44/Pgp-1 antibodies.
- Experiments assessing the effect of treating different cell types with inhibitors.
Main Results:
- Hybridoma cells adhered to hyaluronate and stromal cells, an interaction inhibited by hyaluronidases.
- Exogenous hyaluronate reduced lymphoid cell binding to stromal cells.
- Anti-CD44/Pgp-1 antibodies effectively blocked these adhesive interactions.
Conclusions:
- Hyaluronate recognition by CD44 mediates adhesion between B lineage hybridoma and stromal cells.
- The interaction between hyaluronate and CD44 is critical for cell adhesion in lympho-hemopoiesis.
- Understanding this interaction provides insights into normal hematopoietic processes.