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Updated: May 29, 2026
Method of Studying Palatal Fusion using Static Organ Culture
Published on: September 19, 2015
[Establishment of palatal organ culture in vitro]
Shengjun Lu1, Wei He, Bing Shi
1Dept. of Oral and Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Objective:
The purpose of this study was to establish a palatal organ culture method and to investigate the palatogenesis in vitro.
Methods:
20 pregnant 14-day mice were killed, embryos were separated ascetically, and palatal shelves were dissected and placed on a modified Trowell's system. All explants were cultured 24 h and 48 h respectively. Finally, all explants were embedded and stained by Hematoxylin and Eosin.
Results:
All explants grew healthy. After incubation for 24 h, medial edge epithelium maintained, whereas after 48 h, medial edge epithelium disappeared, bilateral mesenchymal cells contacted, palates fused.
Conclusion:
This method provides an effective way for investigating the etiology of cleft palate in vitro.
