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Updated: May 29, 2026
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
Published on: October 3, 2014
[An animal experiment of construction of functional tissue-engineered bone with cell sheet technology]
Tao Chen1, Yanhui Wang, Lingxue Bu
1Dept. of Oral and Maxillofacial Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China.
Objective:
To construct functional tissue-engineered bone with cell sheet technology and method of traditional bone tissue engineering.
Methods:
Canine bone marrow mesenchymal stem cells (BMSCs) were isolated with the method of density gradient centrifugation and cultured. BMSCs were induced to differentiate into osteoblasts and cultured in temperature-responsive culture dishes at 37 degrees C, 5% CO2 and saturated humidity. BMSCs cell sheet was prepared when temperature was changed to 20 degrees C. Demineralized bone matrix (DBM) and platelet-rich plasma (PRP) were prepared, and complex of DBM/PRP/BMSCs cell sheet/BMSCs was construsted and implanted under the left latissimus dorsi muscle. Complex of DBM/PRP/BMSCs was implanted under the right latissimus dorsi muscle.
Results:
When temperature dropped at 20 degrees C, BMSCs detached automatically from the temperature-responsive culture dishes and formed an intact cell sheet. The osteogenesis of the DBM/PRP/BMSCs cell sheet/BMSCs group was better than that of the DBM/PRP/ BMSCs group.
Conclusion:
Cell sheet technology combined with traditional bone tissue provides a new way for construction of ideal functional tissue-engineered bone.
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