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Updated: May 29, 2026
Synthetic Methodology for Asymmetric Ferrocene Derived Bio-conjugate Systems via Solid Phase Resin-based Methodology
Published on: March 12, 2015
Amplification strategy using aggregates of ferrocene-containing cationic polythiophene for sensitive and specific
Patricia Harding Lepage1, Régis Peytavi, Michel G Bergeron
1Canada Research Chair on Electroactive and Photoactive Polymers, Département de Chimie, Université Laval, Québec City, Québec, Canada.
Abstract:
We report a new electrochemical amplification strategy for an ultrasensitive electrochemical detection of DNA sequences using aggregates composed of a water-soluble, ferrocene-functionalized polythiophene. A two-step hybridization is performed at one addressing surface with PNA capture probes whereas the electrochemical detection is done on an electrode nearby. Specific and quantitative detection of DNA targets with a detection limit of 4 × 10(-16) M (about 4 zeptomoles or about 2500 copies of oligonucleotides) was achieved.
