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Related Experiment Video

Updated: May 29, 2026

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
08:46

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Published on: May 24, 2012

Efficient large volume lentiviral vector production using flow electroporation.

Scott R Witting1, Lin-Hong Li, Aparna Jasti

  • 1Department of Medical and Molecular Genetics, Indiana University School of Medicine , Indianapolis, IN 46202, USA.

Human Gene Therapy
|September 22, 2011
PubMed
Summary
This summary is machine-generated.

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Flow electroporation (EP) offers a scalable, GMP-compliant method for lentiviral vector production in suspension cells. This technique achieves high titers, making it ideal for clinical gene therapy applications.

Area of Science:

  • Biotechnology
  • Gene Therapy
  • Cellular Engineering

Background:

  • Lentiviral vectors are increasingly important for human gene therapy.
  • Current production methods can be challenging for large-scale clinical applications.

Purpose of the Study:

  • To optimize and validate flow electroporation (EP) for scalable lentiviral vector production.
  • To assess the efficiency and safety of flow EP in suspension cell lines for GMP-compliant manufacturing.

Main Methods:

  • Optimized flow electroporation (EP) parameters for serum-free adapted HEK293FT suspension cells.
  • Utilized a third-generation, HIV-based lentiviral vector system with vesicular stomatitis glycoprotein envelope.
  • Performed small- and large-volume transfections to evaluate scalability.

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Lentivirus Production
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Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
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Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

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Last Updated: May 29, 2026

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
08:46

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Published on: May 24, 2012

Lentivirus Production
11:42

Lentivirus Production

Published on: October 2, 2009

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

Main Results:

  • Achieved high lentiviral vector titers exceeding 1×10(8) infectious units/mL.
  • Minimized cell toxicity through optimized flow EP parameters.
  • Demonstrated successful transfections in both small and large volumes.

Conclusions:

  • Flow electroporation (EP) is a highly effective and scalable method for producing lentiviral vectors.
  • This technique is suitable for GMP-compliant, large-scale clinical lentiviral production.
  • Combines suspension cell culture convenience with efficient, non-chemical transfection.