A rapid fluorescence assay for hSMUG1 activity based on modified molecular beacon
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Updated: May 29, 2026
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
Published on: August 6, 2014
Xue Yang1, Chunyi Tong, Ying Long
1College of Biology, Hunan University, Changsha, Hunan 410082, People's Republic of China.
Abstract:
A new method for assay of hSMUG1 in real-time using molecular beacon is reported. hSMUG1 could be detected linearly in the range from 0.67 nM to 10.05 nM and the detection limit is 0.168 nM. In addition, this method was applied to detect the activity of hSMUG1 in tumor cells and study kinetics. The probe with low background signal has been shown to be suitable for the real-time monitoring of hSMUG1 activity, making this a promising method of high-throughput clinical sample analysis.
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