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Related Concept Videos

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
Atomic Emission Spectroscopy: Instrumentation01:22

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The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...

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Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
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Wavelength encoding technique for particle analyses in hematology analyzer.

Nelly Rongeat1, Patrick Brunel, Jean-Philippe Gineys

  • 1HORIBA Medical, Parc Euromédecine, rue du Caducée, 34000 Montpellier Cedex 4, France. nelly.rongeat@horiba.com

Optics Express
|September 22, 2011
PubMed
Summary
This summary is machine-generated.

This study enhances cell fluorescence characterization by combining multiple laser wavelengths for improved accuracy. This method avoids noisy compensation, enabling precise identification of fluorochromes in flow cytometry.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Cell Biology

Background:

  • Accurate fluorescence characterization is crucial for cell analysis.
  • Traditional methods like compensation can introduce noise and inaccuracies.
  • Flow cytometry is a key technique for single-cell analysis.

Purpose of the Study:

  • To improve the accuracy of fluorescence characterization in labeled cells.
  • To develop a method combining multiple excitation wavelengths.
  • To avoid the use of noisy compensation methods in cell analysis.

Main Methods:

  • Utilized a hematology analyzer based on flow cytometry.
  • Employed a continuous wave (CW) laser source emitting two visible wavelengths.
  • Implemented optical encoding for wavelength-specific fluorescence identification.

Main Results:

  • Successfully combined multiple excitation wavelengths for enhanced fluorescence characterization.
  • Demonstrated accurate identification of specific fluorochromes.
  • Showcased the avoidance of noisy compensation methods.

Conclusions:

  • Combining multiple excitation wavelengths improves fluorescence characterization accuracy.
  • Optical encoding provides a robust method for fluorochrome identification.
  • This approach offers a more precise alternative to traditional compensation techniques in flow cytometry.