Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultraviolet irradiation-responsive dynamic ultralong organic phosphorescence in polymeric systems.

Nature communications·2021
Same author

Wavelength beam-combining of terahertz quantum-cascade laser arrays.

Optics letters·2021
Same author

Ultrafast light field tomography for snapshot transient and non-line-of-sight imaging.

Nature communications·2021
Same author

Supercapsular percutaneously-assisted total hip (SuperPath) versus mini-incision posterolateral total hip arthroplasty for hip osteoarthritis: a prospective randomized controlled trial.

Annals of translational medicine·2021
Same author

Association of apolipoproteins A1 and B with type 2 diabetes and fasting blood glucose: a cross-sectional study.

BMC endocrine disorders·2021
Same author

Transgluteal versus prone approach to extracorporeal shockwave lithotripsy for patients with distal ureteral stones: A systematic review and meta-analysis.

Asian journal of surgery·2021

Related Experiment Video

Updated: May 29, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Depth-resolved image mapping spectrometer (IMS) with structured illumination.

Liang Gao1, Noah Bedard, Nathan Hagen

  • 1Department of Bioengineering, Rice University, Houston, Texas 77005, USA.

Optics Express
|September 22, 2011
PubMed
Summary

We developed a depth-resolved Image Mapping Spectrometer (IMS) for 4D datacube acquisition. This novel device achieves superior signal-to-noise ratio compared to hyperspectral confocal microscopy, even with lower light intensity.

More Related Videos

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Published on: September 29, 2014

Related Experiment Videos

Last Updated: May 29, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
12:44

Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)

Published on: September 29, 2014

Area of Science:

  • Optical Imaging
  • Spectroscopy
  • Biomedical Optics

Background:

  • Hyperspectral imaging provides rich spectral information but often lacks depth resolution.
  • Confocal microscopy offers optical sectioning but can be limited in spectral information acquisition.
  • Existing techniques struggle to combine high spectral and depth resolution efficiently.

Purpose of the Study:

  • To introduce a novel depth-resolved Image Mapping Spectrometer (IMS) for acquiring 4D (x, y, z, λ) datacubes.
  • To demonstrate the spectral imaging performance of the IMS in biological samples.
  • To quantitatively compare the IMS with a hyperspectral confocal microscope (HCM).

Main Methods:

  • Development of a depth-resolved Image Mapping Spectrometer (IMS).
  • Implementation of optical sectioning using structured illumination.
  • Acquisition of 4D (x, y, z, λ) datacubes.
  • Fluorescence imaging experiments on multispectral microspheres and mouse kidney tissue.
  • Quantitative comparison with a hyperspectral confocal microscope (HCM).

Main Results:

  • The IMS successfully acquired depth-resolved spectral datacubes.
  • Demonstrated spectral imaging performance in multispectral microspheres and mouse kidney tissue.
  • Achieved a 2.6 times higher image signal-to-noise ratio compared to HCM.
  • The IMS achieved superior performance despite using a light source with four orders of magnitude lower intensity than HCM.

Conclusions:

  • The developed depth-resolved IMS is a powerful tool for 4D spectral imaging.
  • The IMS offers significant advantages in signal-to-noise ratio over conventional hyperspectral confocal microscopy.
  • This technology has potential applications in various fields requiring high-resolution spectral and depth information.