Lipoprint adequately estimates LDL size distribution, but not absolute size, versus polyacrylamide gradient gel...

Recently, a new cost-effective and less labor-intensive technique termed the "lipoprint LDL system" was developed to measure LDL particle size. However, the agreement between lipoprint and previously validated techniques, such as polyacrylamide gradient gel electrophoresis (PGGE), has never been tested. Therefore, we measured LDL size by lipoprint and PGGE in 16 obese subjects at 4 different time points. Lipoprint significantly overestimated (P = 0.003) integrated LDL particle size by 1.1 ± 3.0 Å when compared to PGGE. As for distribution, there was good agreement between methods for the estimation of large, medium, and small particles (mean difference between the methods was <3% for each parameter). Correlational analysis also revealed good relationships between methods for the proportion of large (r = 0.81, P < 0.0001), medium (r = 0.67, P < 0.0001), and small (r = 0.73, P < 0.0001) particles. In sum, although there is good agreement between lipoprint and PGGE for the determination of LDL size distribution, absolute LDL size values may differ between the two methods.