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The effects of methylene blue on adhesion formation in a rat model of experimental peritonitis
Mustafa Uygar Kalaycı1, Hasan Erol Eroğlu, Dilek Kubilay
1Department of General Surgery, Okmeydanı Training and Research Hospital, İstanbul, Turkey.
Background:
We investigated the effects of methylene blue (MB) on the early and late phases of adhesion and abscess formation in a standard colonic wall injury and fecal peritonitis model in rats.
Methods:
There were four groups: Group I (only laparotomy, n=10), Group II (peritonitis + MB, n=15), Group III (peritonitis + saline, n=15), and Group IV (colon incision + saline, n=15). Mortality, morbidity, adhesion scores, histopathologic analyses, serum tumor necrosis factor-alpha (TNF-?) levels, and tissue hydroxyproline (5-HP) levels were evaluated in all animals. Descriptive statistical methods were used with Kruskal-Wallis test. When a statistical difference was obtained between groups, Mann-Whitney U test was used to confirm the difference between two groups.
Results:
Adhesion scores of Groups I, III and IV were significantly higher than in Group II. TNF-? levels were significantly higher in Groups I, III and IV. 5-HP levels were significantly lower in Groups I and II compared to Groups III and IV.
Conclusion:
Based on these results, it appears that MB may prevent peritoneal adhesions in a peritonitis model, but wound healing could be impaired. MB should be further evaluated because of its dual effect.
Insights
Methylene blue (MB) may reduce peritoneal adhesions in a rat peritonitis model. However, MB might also impair wound healing, indicating a dual effect requiring further investigation.
Area of Science:
- Surgical research
- Pharmacology
- Experimental pathology
Background:
- Investigated methylene blue (MB) effects on adhesion and abscess formation.
- Utilized a rat model of colonic wall injury and fecal peritonitis.
Purpose of the Study:
- To evaluate the efficacy of MB in preventing peritoneal adhesions.
- To assess the impact of MB on early and late phases of adhesion and abscess formation.
Main Methods:
- Four groups of rats: control, peritonitis + MB, peritonitis + saline, colon incision + saline.
- Evaluated mortality, morbidity, adhesion scores, histopathology, TNF-α, and 5-HP levels.
- Statistical analysis using Kruskal-Wallis and Mann-Whitney U tests.
Main Results:
- MB group showed significantly lower adhesion scores compared to control groups.
- Tumor necrosis factor-alpha (TNF-α) levels were higher in control groups.
- Tissue hydroxyproline (5-HP) levels were lower in the MB group and one control group.
Conclusions:
- Methylene blue (MB) shows potential in preventing peritoneal adhesions in a peritonitis model.
- MB may have a dual effect, potentially impairing wound healing.
- Further research is warranted to explore the dual effects of MB.
