Related Experiment Video

Updated: May 29, 2026

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
19:44

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Published on: May 30, 2012

Combretastatin A4 phosphate induces programmed cell death in vascular endothelial cells

Xueqiang Ding1, Zhaoqiang Zhang, Su Li

  • 1Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China.

Oncology Research
|September 23, 2011
PubMed

Insights

Combretastatin A4 phosphate (CA4P) effectively targets tumor blood vessels by inducing programmed cell death (PCD) in endothelial cells. This antivascular agent shows promise for treating cancers and hemangiomas.

Area of Science:

  • Vascular biology
  • Cancer research
  • Pharmacology

Background:

  • Combretastatin A4 phosphate (CA4P) is an antivascular agent investigated for cancer therapy.
  • Understanding its mechanism in vascular endothelial cells is crucial for therapeutic development.

Purpose of the Study:

  • To define the antivascular effects of CA4P.
  • To investigate the programmed cell death (PCD) induced by CA4P in vascular endothelial cells.

Main Methods:

  • Assessing antiproliferative activity against human umbilical vein endothelial cells (HUVECs).
  • Evaluating effects on cell cycle progression and DNA fragmentation.
  • Utilizing in vitro wound and tube formation assays to assess endothelial cell migration and vascularization.
  • Measuring apoptosis and autophagy markers, including microtubule-associated protein light chain 3 (LC3)-II.

Main Results:

  • CA4P demonstrated time- and dose-dependent antiproliferative effects on HUVECs.
  • CA4P induced G2/M cell cycle arrest and DNA fragmentation.
  • Significant inhibition of endothelial cell migration and tube formation was observed.
  • Apoptosis and autophagy, indicated by LC3-II induction, were triggered by CA4P treatment.

Conclusions:

  • CA4P exhibits potent antivascular activity by inducing programmed cell death in endothelial cells.
  • CA4P is a promising agent for cancer and hemangioma treatment due to its vascular-targeting properties.

More Related Videos

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog08:37

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog

Published on: November 5, 2014

Related Concept Videos

Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...