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Updated: May 29, 2026

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
Published on: March 16, 2017
Expression and localization of DLX3, PPARG and SP1 in bovine trophoblast during binucleated cell differentiation
S A Degrelle1, P Murthi, D Evain-Brion
1INRA, UMR1198 Biologie du Développement et Reproduction, F-78352 Jouy-en-Josas, France.
Abstract:
The differentiation of the trophoblast is marked in ruminants by the formation of binucleated cells (BNC). They appear from pre-implantation onwards but the molecular mechanisms underlying their differentiation remain largely unexplored. Taking advantage of our recent data, we analyzed the expression pattern of DLX3 and PPARG that are known regulators of early placenta formation and extended our analysis to one of their potential regulators, SP1. Our study is the first to demonstrate the co-expression of DLX3, PPARG and SP1 in bovine BNC nuclei. This suggests a possible role of these transcription factors through BNC specific genes at the time of pre-placental differentiation.
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