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Updated: May 29, 2026

Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ

Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ

Published on: March 22, 2024

The 2',3'-cAMP-adenosine pathway.

Edwin K Jackson¹

¹Dept. of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, 100 Technology Drive, Pittsburgh, PA 15219, USA. edj@pitt.edu.

American Journal of Physiology. Renal Physiology

|September 23, 2011

Summary

This summary is machine-generated.

Researchers discovered 2

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Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ

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Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis

Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis

Published on: October 27, 2020

Area of Science:

Biochemistry
Molecular Biology
Physiology

Background:

The cyclic nucleotide 3',5'-cAMP is a crucial second messenger.
The production and role of other cAMP isomers remain largely unexplored.

Purpose of the Study:

To identify and characterize novel cyclic nucleotide isomers produced by intact kidneys.
To elucidate the metabolic pathway and potential physiological roles of newly discovered cAMP isomers.

Main Methods:

High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) for perfusate analysis.
Nuclear magnetic resonance (NMR) spectroscopy for enzymatic studies.
Isolated, perfused kidney models (rat and mouse) for in vitro and in vivo experiments.

Main Results:

First detection of 2',3'-cyclic adenosine monophosphate (2',3'-cAMP) in kidney perfusate.
Evidence suggests 2',3'-cAMP originates from RNase-mediated mRNA transphosphorylation.
A novel 2',3'-cAMP-adenosine pathway identified: 2',3'-cAMP is metabolized to 2'-AMP/3'-AMP, then to adenosine.
Extracellular 2',3'-cAMP activates mitochondrial permeability transition pores, inhibits cell proliferation, and its levels increase with metabolic poisons.
Adenosine, a product of this pathway, is known to be renoprotective.

Conclusions:

The 2',3'-cAMP-adenosine pathway represents a newly identified biochemical route for adenosine production.
This pathway may play a protective role in acute organ injury, such as acute kidney injury, by detoxifying 2',3'-cAMP and generating protective adenosine.
Further research into the pharmacology of 2',3'-cAMP, 2'-AMP, and 3'-AMP is warranted.