Related Experiment Video
Updated: May 29, 2026

Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells
Published on: November 18, 2022
Oridonin induced the apoptosis of PC-3 cells and its mechanism
Jin Li1, Luoyan Yang, Hongtao Wu
1Department of Urology, Central Hospital of Xiangtan, Xiangtan Hunan 411100,China.
Objective:
To observe the proliferation inhibition and apoptosis promotion effect of oridonin on PC-3 cells.
Methods:
PC-3 cells were treated with different concentrations of oridonin. MTT assay and drug concentration-time survival curve were used to test the effect of oridonin on the PC-3 cells. The percentage of earlier apoptosis cells was analyzed by flow cytometry. The protein expression of caspase-3, Bcl-2, and Bax in the PC-3 cells was detected by Western blot.
Results:
Oridonin effectively inhibited the proliferation of PC-3 cells in both concentration- and time-dependent manner, and the IC50 of PC-3 cells was 10.29 μmol/L. HochesT(3)3258 staining and flow cytometry deteced that oridonin induced the apoptosis of PC-3 cells in a concentration-dependent manner (P<0.05). Oridonin down-regulated Bcl-2, up-regulated Bax protein, and activated caspase-3 in a concentration-dependent manner in the PC-3 cells.
Conclusion:
The apoptosis of PC-3 cells induced by oridonin might be associated with the mitochondrial pathway.
Related Concept Videos
The Intrinsic Apoptotic Pathway
Inhibition of Cdk Activity
The Extrinsic Apoptotic Pathway
Overview of Cell Death
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the 20th century...
Apoptosis
