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[Nerve growth factor increases sodium current via interferon regulatory factor-1 pathway in rat pheochromocytoma
Yemu Zhu1, Pinhua Pan, Hongyi Tan
1Department of Respiratory Medicine, Xiangya Hospital, Central Sounth Univerdity, Changsha 410008, China.
Objective:
To explore the effect of nerve growth factor(NGF) and interferon regulatory factor-1(IRF-1) on sodium current change of sensory neuron in rat pheochromocytoma cells.
Methods:
Sensory neuron rat pheochromocytoma cells were stimulated by different concentrations of NGF(0-200 ng/mL), the IRF-1 mRNA levels were examined by real-time PCR, and the activation of IRF-1 was examined by Western blot. The sodium current change was recorded by patch clamp.
Results:
Low concentration of NGF improved the sodium current, which was concentration dependent. When exposed to high concentration of NGF, the expression of IRF-1 mRNA in PC-12 was improved. Low concentration of NGF resulted in IRF-1 intronuclear transporting, and the expression was not affected. Sodium current did not occur in PC-12 cells when IRF-1 was blocked.
Conclusion:
NGF can improve the sodium current in PC-12 cells concentration-dependently, and the improvement is regulated by IRF-1.
