Related Experiment Video
Updated: May 29, 2026
Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
Published on: September 5, 2019
Intracellular reactive oxygen species production by polymorphonuclear leukocytes in bovine leukemia virus-infected
Fernando Nogueira de Souza1, Maiara Garcia Blagitz, Andréia Oliveira Latorre
1Department of Clinical Science, Faculty of Veterinary Medicine and Animal Sciences, University of São Paulo, Cidade Universitária, São Paulo, SP 05508–270, Brazil. nogueirasouza@yahoo.com.br
Abstract:
The present study assesses the oxidative burst activity from polymorphonuclear leukocytes (PMNLs) from bovine leukemia virus (BLV)-infected cows. Fifteen clinically healthy cows were divided into serologically positive cows without any hematological alteration, serologically positive animals with persistent lymphocytosis (PL) and healthy serologically negative cows. The oxidative burst activity from the PMNLs was evaluated by flow cytometry using 2',7'-dichlorofluorescein diacetate as a probe. PMNLs from each cow were incubated with heat-killed Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) to stimulate oxidative burst activity. The results of the present work showed no significant difference in the oxidative burst activity without any stimulus and elicited by S. aureus. Conversely, a decrease in the oxidative burst index induced by E. coli in PMNLs was observed in BLV-infected cows.
More Related Videos
08:29Measuring Liver Mitochondrial Oxygen Consumption and Proton Leak Kinetics to Estimate Mitochondrial Respiration in Holstein Dairy Cattle
Published on: November 30, 2018
11:00Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli
Published on: April 20, 2021
