Related Experiment Video
Updated: May 29, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
[The detection of isoniazide in the cadaveric blood and plasma by high performance liquid chromatography with a diode
Abstract:
The authors describe the analytical procedure for the rapid detection of isoniazide in the cadaveric blood and plasma using high performance liquid chromatography with a diode matrix detector (HPLC-DAD). The method is designed for toxicological and forensic medical analyses. The preliminary preparation of the samples includes protein precipitation with trichloroacetic acid, formation of isoniazide derivatives with cinnamic aldehyde, and chromatographic analysis on an Eclipse XDB-C18 column with registration of absorption at a wavelength of 340 nm. The mobile phase is a mixture of methanol and 0.05 M ammonium acetate buffer (50:50 v/v), pH 3.8. The detection limit is 0.5 mcg/ml in plasma and 1 mcg/ml in blood hemolizate. The qualitative determination is possible in a concentration range from 2 to 200 mcg/ml. Mean square deviation does not exceed 12% in a concentration interval from 2 to 50 mcg/ml.
More Related Videos
08:54Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples
Published on: May 19, 2020
08:56Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Published on: November 22, 2024
Related Concept Videos
High-Performance Liquid Chromatography: Types of Detectors
Gas Chromatography: Types of Detectors-II
Gas Chromatography: Types of Detectors-I
TCD is the earliest and most widely used detector that operates by measuring the changes in the thermal conductivity of the carrier gas. When a sample compound enters the detector,...
Therapeutic Drug Monitoring: Drug Analysis Methods