Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tail-to-Side-Chain Cyclization Stabilizes Proteins on Thermoresponsive ELP-Based Nanoparticles.

Journal of biotechnology·2026
Same author

Effect of Microwave on Protein Conformations and Enzymatic Reactions.

Molecules (Basel, Switzerland)·2026
Same author

Microwave-induced modulation of intracellular distribution of peptides based on mitochondrial targeting sequences.

Scientific reports·2026
Same author

ssDNA Modification on Lipid Carriers Utilizing ssDNA-Conjugated Fusion Protein with Cholesterol-Binding Ability.

Bioconjugate chemistry·2026
Same author

Comparison of long-term outcomes between proximal gastrectomy and total gastrectomy for advanced gastric cancer in the upper third of the stomach: a propensity score-matched analysis.

Japanese journal of clinical oncology·2026
Same author

Synthesis of biocompatible gold nanoparticles for photothermal therapy by mineralization using peptides.

RSC advances·2025

Related Experiment Video

Updated: May 29, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Cell fingerprint patterns using designed α-helical peptides to screen for cell-specific toxicity.

Kenji Usui1, Takashi Kakiyama, Kin-Ya Tomizaki

  • 1Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. kusui@center.konan-u.ac.jp

Bioorganic & Medicinal Chemistry Letters
|September 24, 2011
PubMed
Summary

We screened alpha-helical peptides using cell fingerprints (CFPs) to predict cytotoxicity. This peptide screening approach identified cell-specific toxic peptides, showing promise for drug discovery.

More Related Videos

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Related Experiment Videos

Last Updated: May 29, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners
14:28

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Identifying Protein-protein Interaction Sites Using Peptide Arrays
07:44

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Peptide-based therapeutics offer targeted treatment strategies.
  • Predicting peptide cytotoxicity remains a challenge in drug development.

Purpose of the Study:

  • To evaluate the utility of cell fingerprints (CFPs) for predicting cytotoxicity of alpha-helical peptides.
  • To identify novel peptides with cell-specific toxic properties.

Main Methods:

  • A library of 101 alpha-helical peptides was synthesized and screened.
  • Cell-based cytotoxicity assays were performed.
  • Cell fingerprints (CFPs) were generated and analyzed to correlate peptide characteristics with toxicity.

Main Results:

  • CFP data indicated a correlation between peptide hydrophobicity, charge, amino acid composition, and cytotoxicity.
  • Several peptides exhibited selective toxicity against specific cell types.
  • The screening strategy successfully identified peptides with cell-specific toxic effects.

Conclusions:

  • Combining designed peptide libraries with CFP analysis is a promising strategy for cell-based peptide screening.
  • CFPs can aid in predicting and understanding peptide-induced cytotoxicity.
  • This approach holds potential for the discovery of targeted peptide therapeutics.