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Isolation and characterization of microsatellite loci in Tsoongiodendron odorum (Magnoliaceae)
Jinghu Jiang1, Jing Wang, Ming Kang
1Key Laboratory of Plant Resources, Conservation, and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong, China.
Premise Of The Study:
Microsatellite loci were developed in Tsoongiodendron odorum, an endangered Magnoliaceae species in subtropical China, for further investigation of its conservation genetics.
Methods And Results:
Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, we isolated and characterized 12 microsatellite loci from T. odorum. Of the samples analyzed, eight were polymorphic and four were monomorphic.
Conclusions:
These characterized markers will enable genetic diversity, gene flow, and mating system studies of T. odorum, which are highly valuable for formulating conservation strategies.
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