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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Alternative splicing at the right time.

Sabrina E Sanchez1, Ezequiel Petrillo, Alberto R Kornblihtt

  • 1Instituto Leloir, IIBBA-CONICET, C1405BWE, Buenos Aires, Argentina.

RNA Biology
|September 24, 2011
PubMed
Summary
This summary is machine-generated.

Alternative splicing (AS) generates diverse mRNA variants from one gene. This review explores how circadian rhythms and epigenetic factors, including PRMT5, regulate AS, offering insights into physiological adaptation.

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Chronobiology

Background:

  • Alternative splicing (AS) increases proteome complexity by producing multiple mRNA variants from a single gene.
  • AS regulation involves splicing factors, spliceosomal machinery, and epigenetic modifications.
  • The precise contribution of these regulatory mechanisms to AS in response to stimuli remains unclear.

Purpose of the Study:

  • To review the link between circadian clocks and alternative splicing.
  • To discuss the role of Protein Arginine Methyltransferase 5 (PRMT5) in circadian-regulated AS.
  • To propose a framework for dissecting AS regulation in a physiological context.

Main Methods:

  • Literature review of studies on circadian rhythms, alternative splicing, and epigenetic regulation.
  • Focus on the role of Protein Arginine Methyltransferase 5 (PRMT5).
  • Synthesis of evidence linking molecular oscillations to splicing regulation.

Main Results:

  • Evidence suggests circadian clocks influence alternative splicing patterns.
  • Protein Arginine Methyltransferase 5 (PRMT5) is implicated in these circadian-AS interactions.
  • Daily rhythms in splicing factors and epigenetic regulators likely contribute to AS control.

Conclusions:

  • Circadian rhythms and alternative splicing are interconnected.
  • PRMT5 plays a role in mediating these connections.
  • Investigating the interplay between circadian rhythms, splicing factors, and epigenetic regulators is crucial for understanding AS regulation.