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Updated: May 29, 2026

Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
Published on: April 6, 2022
Ultraviolet-a light and riboflavin therapy for acanthamoeba keratitis: a case report
Leopoldo Garduño-Vieyra1, Claudia R Gonzalez-Sanchez, Sergio E Hernandez-Da Mota
1Instituto Oftalmologico Privado and Clinica David, Irapuato, Morelia, México.
Purpose:
To report ultraviolet-A (UV-A) light treatment in a patient with Acanthamoeba keratitis (AK).
Methods:
Interventional case report. A standard protocol for ultraviolet corneal therapy, with a power emission of 3 mW/cm(2) and a wavelength of 370 nm, was used. The protocol included an 8-nm bandwidth at a 54-mm distance measured with a collimation system of diodes as well as a protective shield of riboflavin in a case of documented AK.
Results:
A 54-year-old female patient with AK, showing no therapeutic response to a wide variety of topical antimicrobial agents and with a visual acuity of 20/400, was treated with UV-A therapy. The patient displayed a favourable response in the first 24 h after treatment, with improvement of symptoms, visual acuity (to 20/200) and biomicroscopy cornea with haze degree I. By the third week post-treatment, the patient was symptom-free. Her visual acuity was 20/30, and the affected cornea was clear. Five months after treatment, there had been no recurrence, and her vision was 20/20.
Conclusions:
Treatment with UV-A light was an effective therapy in this case of AK.
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