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Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
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UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
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Related Experiment Video

Updated: May 29, 2026

Accuracy in Dental Medicine, A New Way to Measure Trueness and Precision
07:57

Accuracy in Dental Medicine, A New Way to Measure Trueness and Precision

Published on: April 29, 2014

Data comparison between two dental spectrophotometers.

G Khashayar1, A Dozic, C J Kleverlaan

  • 1Academic Centre for Dentistry Amsterdam, Dental Materials, Amsterdam, Netherlands. khashayar@acta.nl

Operative Dentistry
|September 28, 2011
PubMed
Summary
This summary is machine-generated.

Two spectrophotometers for dental shade selection showed low correlation for CIE L*a*b* values. Direct comparison of shade codes was only 40% equal, limiting clinical interchangeability.

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Area of Science:

  • Dental materials science
  • Colorimetry
  • Clinical dentistry

Background:

  • Accurate dental shade selection is crucial for aesthetic restorations.
  • Spectrophotometers are increasingly used to objectively measure tooth color.
  • Comparing data from different devices is essential for clinical workflow integration.

Purpose of the Study:

  • To clinically evaluate the comparability of color data obtained from two different spectrophotometers.
  • To assess the interchangeability of CIE L*a*b* values and shade tab selections between devices.

Main Methods:

  • Two spectrophotometers (Vita Easyshade, Spectro Shade-Micro) measured three tooth areas (cervical, middle, incisal) in 102 participants.
  • Each area was measured thrice for average CIE L*a*b* coordinates and Vita Classical shade tabs.
  • Color differences (ΔE) were calculated using translated Vita tab L*a*b* values.

Main Results:

  • Regression analysis revealed low correlation coefficients for CIE L*a*b* values between the two devices.
  • Direct comparison of shade codes resulted in 40% exact matches and 51% clinical acceptability.
  • Significant discrepancies were observed in color data output.

Conclusions:

  • The two tested spectrophotometers do not provide comparable shade selection outputs.
  • Direct exchange of CIE L*a*b* values between these devices is not recommended.
  • Clinical decisions based on cross-device data may be unreliable.