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A yeast-Escherichia coli shuttle vector containing the M13 origin of replication
N Rhodes1, M Company, B Errede
1Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
Plasmid
|March 1, 1990
Abstract:
A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes.