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A method of the rapid preparation of adenosine 5'-gamma-[32P] triphosphate by chemical synthesis
Preparative Biochemistry
|January 1, 1978
Abstract:
A new chemical method for the synthesis of adenosine 5'-gamma-[32P] triphosphate has been developed based on the reaction of adenosine 5'-diphosphate with ethyl chloroformate. The resulting active mixed anhydride was able to react with [32P]-triethylammonium orthophosphate to give gamma-[32P]ATP.