Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epitope-Resolved Digital SERS Profiling of Structurally Dynamic Antigens via a Multi-Epitope Bispecific Antibody Framework.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Spatially Profiling Trace Cytokine Signatures From Microscopically Derived Skin Samples to Probe Skin Disease Inflammation.

Small methods·2026
Same author

Multifactor authentication in extracellular vesicle analysis: methods and approaches to address the heterogeneity problem.

Nature methods·2026
Same author

Glycaemic variability underlies myocyte dysfunction and myocardial injury risk in diabetes.

Nature communications·2026
Same author

Nanobiosensors and Artificial Intelligence Strategies for Glycan Profiling in Cancer Progression: A Critical Review.

ACS sensors·2026
Same author

Magnetoelectrically Enhanced Molecular Recognition on Plasmonic Surfaces.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Heterojunction-Enhanced Interfacial Evanescent-Tunable Fiber Optic Probe for Amplification-free CRISPR/Cas12a-Based Rapid and Ultrasensitive Detection of MPXV.

Analytical chemistry·2026
Same journal

Tunable Charge Transfer in Europium Metal-Organic Frameworks for Ratiometric Sensing of a Sarin Simulant.

Analytical chemistry·2026
Same journal

A β-Cyclodextrin/Ag<sub>2</sub>O@MWCNT-Based Stochastic Platform for the Simultaneous Molecular Enantiorecognition and Enantioanalysis of Twelve Amino Acids in Biological Matrices.

Analytical chemistry·2026
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: May 29, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

Sensitive quantification of somatic mutations using molecular inversion probes.

Rena Hirani1, Ashley R Connolly, Lisa Putral

  • 1Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD, Australia.

Analytical Chemistry
|September 28, 2011
PubMed
Summary
This summary is machine-generated.

A new multiplex assay detects rare DNA mutations in cancer cells, even with minimal tissue samples. This advance aids in sensitive cancer biomarker discovery and targeted therapy selection.

More Related Videos

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
10:57

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Published on: August 14, 2018

Related Experiment Videos

Last Updated: May 29, 2026

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
10:57

Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay

Published on: August 14, 2018

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Somatic DNA mutations are key cancer biomarkers for treatment selection.
  • Detecting these mutations in limited tissue biopsies is challenging due to low mutation levels and wild-type allele interference.

Purpose of the Study:

  • To develop a sensitive and specific multiplex assay for DNA mutation analysis in small cell populations.
  • To enable the detection of nucleotide changes present at very low frequencies within tissue samples.

Main Methods:

  • Developed a multiplex assay utilizing molecular inversion probes.
  • Extended probe capabilities for sensitive discrimination and quantification of low-frequency mutations.
  • Incorporated flow cytometry for rapid readout using multiplexable DNA biosensors.

Main Results:

  • Successfully detected nucleotide changes in less than 150 cells.
  • Achieved sensitive discrimination and quantification of mutations present in less than 0.1% of a cell population.
  • Validated the assay by detecting KRAS gene mutations, common in many cancers.

Conclusions:

  • The developed multiplex assay significantly enhances the ability to detect rare somatic mutations in tissue samples.
  • This method facilitates sensitive biomarker analysis for guiding cancer treatment decisions.
  • The assay offers a powerful tool for precision oncology research and clinical applications.