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Updated: May 29, 2026
A Murine Model of Stent Implantation in the Carotid Artery for the Study of Restenosis
Published on: May 14, 2013
[The role of micro-RNA/143/145 in evolution of intra-stent restenosis]
Abstract:
The mechanisms of neointima formation and hyperplasia in restenosis remain non-elucidated yet. Because micro-ribonucleic acids/143/145(micro-RNA/143 and micro-RNA/145) participate in the regulation and sustaining of the genotype of mature vascular myocytes we have measured their expression in tissue content of restenoses taken postmortem from 5 patients who underwent angioplasty and subsequently died, and studied its association with actin quantity and fibrillar collagen type I degradation degree. It has been found that during restenosis progression quantity of micro-RNA/143 and micro-RNA/145 decreases in media and intima of coronary artery. This finding has been associated with appearance in coronary intima of coronary myocytes with reduced size likely of secretory phenotype, diminution of number of myocytes with contractile phenotype, and increase of quantity of denaturized collagen type I-phenomena characteristic for neointima hyperplasia, a substrate of intra-stent restenosis.
Insights
Micro-ribonucleic acids (microRNAs) 143 and 145 decrease during restenosis progression. This reduction is linked to changes in vascular myocytes and collagen, contributing to neointima hyperplasia after angioplasty.
Area of Science:
- Cardiovascular Biology
- Molecular Medicine
- Genetics
Context:
- Restenosis, a complication of angioplasty, involves neointima hyperplasia.
- The precise mechanisms driving neointima formation are not fully understood.
- Micro-ribonucleic acids (microRNAs) are implicated in vascular cell regulation.
Purpose:
- To investigate the expression of micro-ribonucleic acid 143 (microRNA-143) and micro-ribonucleic acid 145 (microRNA-145) in restenotic tissues.
- To correlate microRNA expression with changes in vascular myocytes and collagen type I.
- To elucidate the role of these microRNAs in the pathogenesis of post-angioplasty restenosis.
Summary:
- Expression levels of microRNA-143 and microRNA-145 were found to decrease in the media and intima of coronary arteries during restenosis.
- This downregulation correlated with the presence of smaller, potentially secretory vascular myocytes and a decrease in contractile myocytes.
- Increased denaturation of collagen type I was also observed, characteristic of neointima hyperplasia.
Impact:
- Provides novel insights into the molecular mechanisms underlying restenosis and neointima hyperplasia.
- Identifies potential roles for microRNA-143 and microRNA-145 in vascular remodeling.
- Suggests these microRNAs could be therapeutic targets for preventing or treating intra-stent restenosis.
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