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Related Concept Videos

Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
Introduction to Enzymes01:22

Introduction to Enzymes

The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that bind the substrates and convert them into products. Many enzymes also...
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

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Related Experiment Video

Updated: May 23, 2026

A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

EnzymeDetector: an integrated enzyme function prediction tool and database.

Susanne Quester1, Dietmar Schomburg

  • 1Institute of Bioinformatics and Biochemistry, Technische Universität Braunschweig, Braunschweig, Germany. S.Quester@tu-bs.de

BMC Bioinformatics
|September 28, 2011
PubMed
Summary
This summary is machine-generated.

EnzymeDetector identifies inconsistencies in enzyme function predictions across major databases. This tool aids researchers by providing a reliable, consolidated view of enzymatic functions for genomes.

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Last Updated: May 23, 2026

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08:10

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Area of Science:

  • Genomics
  • Bioinformatics
  • Enzymology

Background:

  • Accurate prediction of enzymatic functions is crucial for understanding cellular processes and metabolic modeling.
  • Existing biological databases often present inconsistent genome annotations.
  • Inconsistencies in enzyme predictions can hinder biological research and model reconstruction.

Purpose of the Study:

  • To address the challenge of inconsistent genome annotations in enzyme function prediction.
  • To develop a tool that automatically compares, evaluates, and supplements enzyme function predictions.
  • To provide a reliable resource for researchers needing accurate enzymatic function data.

Main Methods:

  • Analysis of nine prokaryotic genomes revealed approximately 70% inconsistencies in enzyme predictions.
  • Implementation of the EnzymeDetector annotation pipeline.
  • EnzymeDetector integrates sequence similarity analysis, organism-specific data from BRENDA (Braunschweig Enzyme Database), and sequence pattern searches.

Main Results:

  • EnzymeDetector offers a rapid and thorough overview of genome-wide enzyme function annotations.
  • The tool highlights annotation errors present in single databases through a relevance score.
  • User-customizable weighting schemes and cut-offs allow for tailored annotation quality assessment.

Conclusions:

  • EnzymeDetector provides a valuable, consolidated resource for enzymatic function annotation.
  • The web interface facilitates easy application of custom quality criteria and data download.
  • Results are stored in a database accessible at http://enzymedetector.tu-bs.de, ensuring up-to-date information.