The indirect fluorescent antibody test for bovine anaplasmosis

An indirect fluorescent antibody test was used successfully for the serodiagnosis of experimental Anaplasma infections in cattle. Specific antibodies were detected three to ten days after anaplasma bodies were found in the blood, and persisted at least 15 weeks post-infection. An American and an African stock of A. marginale were used to prepare antigens, and gave comparable results when tested on sera positive to either of these stocks, as well as to an A. central-like stock from Korea. There were no cross-reactions with several Theileria, Babesia, Trypanosoma and Eperythrozoon species.