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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...

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Related Experiment Video

Updated: May 29, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
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Published on: February 27, 2020

Quantification of proteins and their modifications using QconCAT technology.

Kathleen M Carroll1, Francesco Lanucara, Claire E Eyers

  • 1Manchester Centre for Integrative Systems Biology, University of Manchester, Manchester Interdisciplinary Biocentre, Manchester, United Kingdom.

Methods in Enzymology
|September 28, 2011
PubMed
Summary

Designer QconCAT proteins enable precise quantification of protein components and their post-translational modifications in biological systems. This method is crucial for building accurate mathematical models and testing scientific hypotheses in yeast and mammalian cell extracts.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

  • Proteomics
  • Systems Biology
  • Biochemistry

Background:

  • Mathematical modeling of biological systems requires precise quantification of components.
  • Protein post-translational modifications (PTMs) add complexity, necessitating individual quantification of modified forms.
  • Accurate PTM quantification is vital for understanding protein function and biological pathways.

Purpose of the Study:

  • To present a method for quantifying absolute amounts of polypeptide components and their PTMs.
  • To demonstrate the utility of designer QconCAT proteins in complex biological samples.
  • To facilitate the development of more accurate mathematical models of cellular processes.

Main Methods:

  • Utilized designer QconCAT proteins as internal standards.
  • Applied quantitative mass spectrometry for absolute protein quantification.
  • Analyzed protein and PTM levels in yeast and mammalian cell extracts.

Main Results:

  • Successfully determined absolute quantities of specific protein components.
  • Quantified various covalently modified forms of proteins.
  • Demonstrated the applicability of the method across different species (yeast and mammalian).

Conclusions:

  • Designer QconCAT proteins provide a robust approach for quantifying proteins and their PTMs.
  • This methodology enhances the accuracy of data input for biological system modeling.
  • Enables precise hypothesis testing in systems biology research.