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Kinetics of protease hydrolysis of extended peptide substrates: measurement by flow-injection analysis
G L Chong1, L C Davis, G R Reeck
1Department of Biochemistry, Kansas State University, Manhattan 66506.
Analytical Biochemistry
|May 1, 1990
Abstract:
A flow-injection analysis (FIA) system was developed to study the enzyme-catalyzed hydrolysis of synthetic peptides, each of which contained one scissile bond. The concentrations of alpha-amino groups in reactions mixtures were determined by FIA with o-phthalaldehyde as a fluorescence reagent. The method allows a rapid, precise, and sensitive determination of kinetic constants for proteases acting on extended peptide substrates.