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Updated: May 29, 2026
09:16
Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
Published on: May 3, 2014
New aspects of RNA processing in prokaryotes.
Elena Evguenieva-Hackenberg1, Gabriele Klug
1Institut für Mikrobiologie und Molekularbiologie, University of Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany. Elena.Evguenieva-Hackenberg@mikro.bio.uni-giessen.de
Current Opinion in Microbiology
|September 28, 2011
Summary
Posttranscriptional gene regulation is crucial in prokaryotes, influenced by ribonucleases, regulatory RNAs, and RNA features. Genome-wide studies reveal insights into mRNA stability and decay mechanisms.
Area of Science:
- Molecular Biology
- Genetics
- Microbiology
Background:
- Genome-wide analyses reveal a low correlation between mRNA and protein levels in prokaryotes, highlighting the importance of posttranscriptional gene regulation.
- mRNA stability and translation availability are key determinants of gene expression, influenced by cellular factors.
Purpose of the Study:
- To explore the molecular mechanisms governing mRNA decay in prokaryotes.
- To comprehensively map processed transcripts and regulatory RNAs using high-resolution transcriptome analyses.
Main Methods:
- Genome-wide transcriptome analyses
- High-resolution mapping of processed transcripts
- Detection of regulatory RNAs
- Monitoring the impact of ribonucleases and processing factors
Main Results:
- Identification of key factors influencing mRNA stability: ribonucleases, regulatory RNAs, and RNA sequence/structure.
- Comprehensive mapping of prokaryotic transcriptomes, including processed transcripts and regulatory RNAs.
- Global impact assessment of ribonucleases on mRNA decay pathways.
Conclusions:
- Posttranscriptional gene regulation plays a pivotal role in prokaryotic gene expression.
- High-resolution transcriptome analysis provides novel insights into mRNA decay mechanisms.
- Understanding these mechanisms is essential for deciphering gene regulation in bacteria and archaea.
Related Concept Videos
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
02:01Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
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Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
01:35Pre-mRNA Processing: Modification of pre-mRNA Ends
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...