Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reframing cancer drug resistance: transporters, persister cells, senescence, and emerging therapeutic strategies.

Magyar onkologia·2026
Same author

A human corticospinal organoid-slice connectoid model informs enhancer strategies for post-injury axon regrowth.

Cell reports·2026
Same author

Early α-synuclein-mediated mitochondrial dysfunction in a human cell model of Parkinson's disease dementia.

Communications biology·2026
Same author

Female metabolic resilience and male-biased protein quality defects under hypoxia in human brain organoids.

iScience·2026
Same author

Podocin oligomers regulate the ordering of nephrin chains, providing the molecular basis of <i>NPHS2</i> interallelic interactions.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Quantitative analysis of DNA-GATA1 binding alterations linked to hematopoietic disorders.

The FEBS journal·2026

Related Experiment Video

Updated: May 29, 2026

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

Calcium signaling in pluripotent stem cells.

Ágota Apáti1, Katalin Pászty, Zsuzsa Erdei

  • 1Membrane Research Group of the Hungarian Academy of Sciences, Semmelweis University, Budapest, Hungary.

Molecular and Cellular Endocrinology
|September 28, 2011
PubMed
Summary

This study explores calcium signaling in human embryonic stem cells (hESC) and their derivatives. Researchers found specific ligands trigger distinct calcium responses, offering insights into stem cell differentiation and function.

More Related Videos

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation
11:38

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation

Published on: October 4, 2024

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
06:42

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: May 26, 2023

Related Experiment Videos

Last Updated: May 29, 2026

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation
11:38

A Live-cell Image-Based Machine Learning Strategy to Monitor Pluripotent Stem Cell Differentiation

Published on: October 4, 2024

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
06:42

Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: May 26, 2023

Area of Science:

  • Stem cell biology
  • Cellular signaling
  • Developmental biology

Background:

  • Pluripotent stem cells offer novel avenues for studying cell development and differentiation.
  • Calcium signaling in pluripotent stem cells and their derivatives remains underexplored due to culturing and methodological challenges.
  • Existing literature on calcium signaling in stem cells has potential limitations.

Purpose of the Study:

  • To review current data on calcium signaling in pluripotent stem cells.
  • To discuss limitations of previous studies and survey assay methods.
  • To investigate calcium signaling modulation in human embryonic stem cells (hESC) and their derivatives.

Main Methods:

  • Utilized fluorescent calcium indicator Fluo-4 and confocal microscopy.
  • Examined calcium signaling in HUES9 (hESC), mesenchymal stem cell-like (MSCl) cells, and stem cell-derived cardiomyocytes.
  • Tested responses to various ligands including LPA, trypsin, angiotensin II, histamine, thrombin, ATP, GABA, adrenaline, and verapamil.

Main Results:

  • LPA, trypsin, and angiotensin II induced calcium signals in both HUES9 and MSCl cells.
  • Histamine and thrombin selectively activated calcium signals in MSCl cells; ATP activated signals only in HUES9 cells.
  • Adrenaline increased beating rate and calcium peaks in cardiomyocytes, while verapamil decreased calcium and stopped contractions.

Conclusions:

  • Specific ligands elicit differential calcium responses in hESC and their derivatives, highlighting cell-type specific signaling.
  • The findings provide a foundation for understanding calcium's role in stem cell differentiation and function.
  • This research addresses methodological challenges and offers insights into reliable calcium measurement techniques for stem cells.