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Updated: May 29, 2026
Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
[Development of a time-resolved fluoroimmunoassay for detecting S1 subunit of pertussis toxin and its application]
Qiang Ma1, Guan-feng Lin, Li-ping Zou
1Institute of Antibody Engineering, Southern Medical University, Guangzhou, China. mqqqm2006@163.com
Objective:
To develop a time-resolved fluoroimmunoassay (TRFIA) for detection of pertussis toxin (PT) S1 subunit for quality control of human PT vaccine.
Methods:
A double antibody sandwich one-step method was used to establish the TRFIA for detecting PT S1 subunit in the vaccine.
Results:
The sensitivity of c peptide analysis reached 2.5 ng/ml without cross-reactions with other antigens. This assay could be used in detecting S1 subunit in the vaccine.
Conclusion:
The TRFIA for detecting PT S1 subunit is simple, sensitive and rapid for quality control of the PT vaccine.
