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Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

Loading solution prevents activation damage of human platelets before lyophilization.

Jun Zhou¹, Changhong Zhang, Jinghan Liu

¹Department of Transfusion, Beijing Military Area General Hospital, Beijing, China. howlett@yeah.net

|September 28, 2011

Summary

This study optimizes platelet lyophilization by identifying key inhibitors like prostaglandin E1 (PGE1) and L-arginine. The best combination preserves platelet function and prevents activation damage during freeze-drying.

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Published on: December 7, 2012

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Area of Science:

Biotechnology
Hematology
Biomedical Engineering

Background:

Platelet preservation for transfusion and research is challenging due to activation and aggregation.
Lyophilization offers a stable storage method but requires optimized protective solutions to maintain platelet viability and function.

Purpose of the Study:

To optimize inhibitor compositions in loading solutions for platelet lyophilization.
To establish pretreatment methods for effective platelet freeze-drying.

Main Methods:

Orthogonal experimental design was used to analyze six platelet activation inhibitors: prostaglandin E1 (PGE1), adenosine, L-arginine, phyticacid, bivalirudin, and cilostazol.
Platelet activation was assessed by measuring p-selectin (CD62p), platelet membrane glycoprotein (PAC-1) expression, and mean platelet volume (MPV).
Platelet reactivity and aggregation function were evaluated using thrombin-induced activation, maximal aggregation, and slide platelet aggregation test (SPAT).

Main Results:

Level I loading conditions did not adversely affect MPV, CD62p, PAC-1, SPAT, or maximum platelet aggregation rate.
Level II of PGE1, L-arginine, phycicacid sodium, and Bivalirudin effectively inhibited platelet activation, preserving function.
The optimal loading solution comprised plasma with 1 μM PGE1, 5 mM L-arginine, 0.5 mM phyticacid, and 0.5 μM bivalirudin.

Conclusions:

The optimized loading solution effectively prevents platelet activation damage prior to lyophilization.
This formulation maintains critical platelet functions, enhancing the potential for stable, long-term storage.
The study establishes a viable pretreatment method for platelet lyophilization.