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Related Concept Videos

Disorders of the Skeletal Muscle01:28

Disorders of the Skeletal Muscle

The clinical conditions affecting the skeletal muscle tissue are broadly categorized as musculoskeletal and neuromuscular disorders.
Musculoskeletal disorders
Musculoskeletal disorders involve injuries and conditions affecting the skeletal muscles and associated connective tissues. These disorders can arise from acute biomechanical stresses or chronic overuse and can occur across different age groups. Common injuries include sprains, fractures, and muscular strains, often resulting from...
Skeletal Muscle Anatomy00:55

Skeletal Muscle Anatomy

Skeletal muscle is the most abundant type of muscle in the body. Tendons are the connective tissue that attaches skeletal muscle to bones. Skeletal muscles pull on tendons, which in turn pull on bones to carry out voluntary movements.
Overview of Muscle Tissues01:25

Overview of Muscle Tissues

The human body has three types of muscle tissue: skeletal, smooth, and cardiac. Each class has unique properties that enable them to perform specific functions. However, all muscle tissues share certain properties, including elasticity, contractility, and excitability. 
Elasticity
Elasticity is the ability of muscles to stretch and return to their original shape. This property is partly due to elastic fibers — macromolecules that run through the muscles. These fibers are firm and resilient,...
Classification of Skeletal Muscle Fibers01:48

Classification of Skeletal Muscle Fibers

Skeletal muscles continuously produce ATP to provide the energy that enables muscle contractions. Skeletal muscle fibers can be categorized into three types based on differences in their contraction speed and how they produce ATP, as well as physical differences related to these factors. Most human muscles contain all three muscle fiber types, albeit in varying proportions.
Slow-Twitch Muscle Fibers
Slow oxidative, muscle fibers appear red due to large numbers of capillaries and high levels of...
Satellite Stem Cells and Muscular Dystrophy01:21

Satellite Stem Cells and Muscular Dystrophy

Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
Gross Anatomy of Skeletal Muscles01:12

Gross Anatomy of Skeletal Muscles

The connective tissues play a significant role in arranging the muscle fibers into a hierarchical structure that forms a complete muscle. Consider a muscle like the bicep brachii, commonly called the bicep. This muscle comprises thousands of muscle fibers enclosed by a protective layer of connective tissue called the endomysium. The endomysium is primarily composed of reticular fibers, a type of thin collagen fiber. It allows the exchange of nutrients and waste products at the fiber level,...

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Related Experiment Video

Updated: May 29, 2026

Immunolabelling Myofiber Degeneration in Muscle Biopsies
06:37

Immunolabelling Myofiber Degeneration in Muscle Biopsies

Published on: December 5, 2019

[ABC in muscle pathology].

Ichizo Nishino1

  • 1Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry.

Rinsho Shinkeigaku = Clinical Neurology
|September 28, 2011
PubMed
Summary
This summary is machine-generated.

Proper muscle biopsy handling and staining techniques are crucial for diagnosing muscle diseases. Various stains reveal specific abnormalities, aiding in differentiating myopathic from neuropathic conditions.

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Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

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Last Updated: May 29, 2026

Immunolabelling Myofiber Degeneration in Muscle Biopsies
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Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation
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Collection of Skeletal Muscle Biopsies from the Superior Compartment of Human Musculus Tibialis Anterior for Mechanical Evaluation

Published on: September 27, 2020

Area of Science:

  • Neurology
  • Pathology
  • Histology

Context:

  • Muscle biopsy is essential for diagnosing neuromuscular disorders.
  • Accurate specimen handling and fixation prevent artifacts.
  • Histopathological analysis provides critical diagnostic information.

Purpose:

  • To outline the importance of proper muscle biopsy procedures.
  • To describe key histochemical stains used in muscle pathology.
  • To explain how different stains aid in diagnosing muscle diseases.

Summary:

  • Proper muscle biopsy handling and fixation are vital to prevent artifacts.
  • Hematoxylin and eosin staining reveal basic morphological changes like necrosis and fibrosis.
  • Special stains like Modified Gomori trichrome, NADH-tetrazolium reductase, and Myosin ATPase identify specific structural abnormalities, protein aggregates, myofibrillar disorganization, and fiber types.

Impact:

  • Standardized techniques ensure reliable diagnostic results.
  • Histochemical stains facilitate the differentiation between myopathic and neuropathic processes.
  • Comprehensive analysis of muscle biopsy findings leads to accurate diagnosis and patient management.