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Updated: May 29, 2026
Experimental Strategies to Bridge Large Tissue Gaps in the Injured Spinal Cord after Acute and Chronic Lesion
Published on: April 5, 2016
Joint use of a chitosan/PLGA scaffold and MSCs to bridge an extra large gap in dog sciatic nerve
1Nantong University, Nantong, People's Republic of China.
Background:
Tissue-engineered nerve grafts (TENGs) constitute a promising alternative to nerve autografts that are recognized as the gold standard for surgical repair of peripheral nerve gaps.
Objective:
To investigate the feasibility of using TENGs for bridging extra large peripheral nerve gaps in large animals.
Methods:
TENGs were constructed by incorporating autologous bone marrow mesenchymal stem cells (MSCs) into a neural scaffold that consisted of a chitosan conduit inserted with poly(lactic-co-glycolic acid) (PLGA) fibers. A 60-mm-long sciatic nerve gap in dogs was bridged by TENGs, chitosan/PLGA scaffolds, or nerve autografts. At 12 months postsurgery, behavioral analysis, electrophysiology, retrograde fluorogold tracing, and histological examination were performed.
Results:
The outcomes of TENGs were similar to those of autografts and better than those of scaffolds alone.
Conclusion:
Introduction of autologous MSCs to a chitosan/PLGA scaffold improved the repair and rehabilitation of a large gap after peripheral nerve injury in dogs. Autologous MSCs may be a source of support cells for neural tissue engineering.
