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Updated: May 29, 2026
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A Quantitative Glycomics and Proteomics Combined Purification Strategy
Published on: March 8, 2016
N-linked global glycan profiling by nanoLC mass spectrometry
Michael S Bereman1, David C Muddiman
1Department of Genome Sciences, University of Washington, Seattle, WA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|September 28, 2011
Abstract:
A method is detailed for the global profiling of underivatized N-linked glycans that are derived from complex protein mixtures. The method consists of five main steps that include the following: (1) protein denaturation; (2) enzymatic digestion; (3) solid phase extraction; (4) nanoLC MS analysis; and (5) data interpretation. Materials, methods, and algorithms for the identification of both glycan composition and structure are summarized. In addition, potential problems and their resolutions are addressed.
