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Related Experiment Video

Updated: May 29, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
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Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

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Functionalized soluble nanopolymers for phosphoproteome analysis.

Anton Iliuk1, Keerthi Jayasundera, Rachel Schluttenhofer

  • 1Department of Biochemistry, Purdue Center for Cancer Research, Purdue University, West Lafayette, IN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 28, 2011
PubMed
Summary
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We developed PolyMAC, a novel nanopolymer technique for efficiently isolating phosphopeptides. This method enhances phosphoproteomic analysis by improving the capture of crucial signaling molecules from complex biological samples.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Protein phosphorylation is a key regulator of cellular processes.
  • Phosphoproteomics, often using mass spectrometry, studies these phosphorylation-dependent networks.
  • Efficient phosphopeptide enrichment is crucial for successful phosphoproteomic analysis.

Purpose of the Study:

  • To develop a novel and effective method for phosphopeptide enrichment.
  • To improve the efficiency and accuracy of phosphoproteomic analyses.
  • To introduce a soluble nanopolymer-based technique for phosphopeptide isolation.

Main Methods:

  • Development of a soluble nanopolymer-based phosphopeptide enrichment technique, termed PolyMAC (polymer-based metal ion affinity capture).
  • Utilizing the homogenous and hyperfunctional properties of the PolyMAC reagent for phosphopeptide binding.

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A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
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Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
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Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

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Last Updated: May 29, 2026

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
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Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
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A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

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  • Application and validation of the PolyMAC technique using simple protein mixtures and complex cell extracts.

    • Main Results:

    • PolyMAC demonstrated highly efficient phosphopeptide binding and isolation.
    • The technique proved effective in enriching phosphopeptides from complex biological samples.
    • Successful application in both simple protein mixtures and complex cell extracts confirmed PolyMAC's utility.

    Conclusions:

    • PolyMAC is a novel, effective, and competent technique for phosphopeptide enrichment.
    • This nanopolymer-based method significantly enhances phosphoproteomic analysis.
    • PolyMAC offers a superior alternative for isolating phosphopeptides from complex biological samples.