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Updated: May 29, 2026
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Improving Student Outcomes with an Adaptable Molecular Cloning Course-Based Undergraduate Research Experience
Published on: November 15, 2024
The "Frankenplasmid" lab: an investigative exercise for teaching recombinant DNA methods
1Department of Biology, Williams College, Williamstown, Massachusetts 01267, USA. ddean@williams.edu
Summary
This lab module provides undergraduates hands-on experience with recombinant DNA technology, enabling them to map unknown "Frankenplasmids" using molecular biology techniques like PCR and restriction mapping.
Area of Science:
- Molecular Biology
- Biotechnology Education
Background:
- Undergraduate science education requires practical laboratory experiences.
- Recombinant DNA techniques are fundamental in modern molecular biology.
Purpose of the Study:
- To develop and assess an investigative laboratory module for teaching recombinant DNA methods.
- To provide college undergraduates with practical and theoretical experience in molecular cloning and plasmid mapping.
Main Methods:
- Students deduce restriction enzyme maps of plasmids.
- Ligation of plasmids, transformation of E. coli, and selection of hybrid plasmids.
- Gene mapping of hybrid plasmids using LacZ phenotype, PCR, and restriction mapping.
Main Results:
- Students successfully generated and mapped novel hybrid plasmids.
- The protocol accommodates multiple possible outcomes, simulating real research scenarios.
- The module effectively teaches recombinant DNA procedures through an investigative approach.
Conclusions:
- The investigative module provides effective, engaging training in recombinant DNA techniques.
- The modular design allows for adaptability to various course structures and schedules.
- This hands-on approach enhances student understanding and problem-solving skills in molecular biology.
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