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Related Concept Videos

Renal Corpuscle01:20

Renal Corpuscle

The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous capillaries...
Glucose Transporters01:27

Glucose Transporters

Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Diabetic Nephropathy01:28

Diabetic Nephropathy

Definition Diabetic nephropathy is a chronic kidney complication that results from prolonged hyperglycemia.Prevalence It is the most common cause of chronic kidney disease (CKD) and end-stage renal disease (ESRD) worldwide, affecting up to half of individuals with diabetes.Pathophysiology • Sustained hyperglycemia triggers multiple hemodynamic and metabolic changes in the kidney. • Early in the disease, increased renal blood flow and glomerular hyperfiltration occur due to afferent arteriolar...
Nephrotic Syndrome I : Introduction01:24

Nephrotic Syndrome I : Introduction

Nephrotic Syndrome is a chronic kidney disorder defined by clinical findings such as severe proteinuria, hypoalbuminemia, hyperlipidemia, and edema. These symptoms result from damage to the glomeruli, the kidney’s filtering units, increasing their permeability to proteins.Definition and Meaning:Proteinuria, defined as the loss of more than 3.5 grams of protein per day in adults, is a crucial feature of nephrotic syndrome. This condition is often accompanied by edema, the accumulation of fluid...
Overview of Protein Metabolism01:21

Overview of Protein Metabolism

Proteins are broken down into amino acids during digestion. Unlike fats and carbohydrates, which are stored for later use, proteins are not. Instead, amino acids are either used to produce ATP through oxidation or contribute to the creation of new proteins for the growth and repair of the body. Any surplus amino acids from the diet are converted into glucose or triglycerides rather than excreted.
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
Inborn Errors of Metabolism01:20

Inborn Errors of Metabolism

Phenylketonuria (PKU) is a protein metabolism disorder characterized by high blood levels of the amino acid phenylalanine. This results from a mutation in the gene responsible for phenylalanine hydroxylase, an enzyme that converts phenylalanine into tyrosine. When this enzyme is deficient, phenylalanine builds up in the blood, leading to symptoms such as vomiting, rashes, seizures, growth deficiency, and severe mental retardation. An early diagnosis and a diet restricting phenylalanine intake...

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Related Experiment Video

Updated: May 29, 2026

Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
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Glcci1 deficiency leads to proteinuria.

Yukino Nishibori1, Kan Katayama, Mataleena Parikka

  • 1Department of Medical Biochemistry and Biophysics, Division of Matrix Biology, Karolinska Institutet, 171 77 Stockholm, Sweden.

Journal of the American Society of Nephrology : JASN
|September 28, 2011
PubMed
Summary

Glucocorticoid-induced transcript 1 (GLCCI1) is crucial for maintaining podocyte structure and glomerular filtration barrier function. This study reveals GLCCI1

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Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

Area of Science:

  • Nephrology
  • Developmental Biology
  • Molecular Biology

Background:

  • Glucocorticoid-induced transcript 1 (GLCCI1) is a glomerulus-specific transcript.
  • Its function in glomerular development and disease remains largely unknown.

Purpose of the Study:

  • To investigate the role of GLCCI1 in glomerular development and podocyte function.

Main Methods:

  • RT-PCR and Western blotting in mouse kidneys.
  • Immunohistochemistry for protein localization.
  • Zebrafish gene knockdown using morpholinos.
  • Glomerular permeability studies.

Main Results:

  • Mouse glomeruli exhibit high Glcci1 protein levels, localized to podocyte cytoplasm.
  • Intense Glcci1 expression is observed during the capillary-loop stage of fetal kidney development.
  • Zebrafish lacking Glcci1 function developed collapsed glomeruli with foot-process effacement.
  • Glcci1 knockdown disrupted the glomerular filtration barrier's selective permeability.

Conclusions:

  • GLCCI1 is essential for normal podocyte development and structural integrity.
  • GLCCI1 plays a critical role in maintaining the function of the glomerular filtration barrier.
  • These findings highlight GLCCI1 as a key factor in glomerular health and disease.