Related Experiment Video
Updated: May 29, 2026

Ecotoxicological Methodologies to Evaluate Biomarkers at Different Scales in Neotropical Anurans
Published on: April 28, 2023
Biomarkers of exposure and effect-interpretation in human risk assessment
Radim J Sram1, Blanka Binkova, Olena Beskid
1Institute of Experimental Medicine, Academy of Sciences of the Czech Republic (ASCR), 142 20 Prague, Czech Republic.
Abstract:
The effect of exposure to carcinogenic polycyclic aromatic hydrocarbons adsorbed onto respirable air particles (PM2.5, diameter < 2.5 μm) on DNA adducts and chromosomal aberrations was repeatedly studied in Prague, Czech Republic, in groups of policemen working in the downtown area and in bus drivers. Personal exposure was evaluated using personal samplers during working shifts. DNA adducts were analyzed in lymphocytes by the (32)P-postlabeling assay and chromosomal aberrations were analyzed by conventional cytogenetic analysis and fluorescent in situ hybridization (FISH). The impact of environmental pollution on DNA adducts and chromosomal aberrations was studied in a total of 950 subjects. Our results suggest that the environmental exposure of nonsmokers to concentrations higher than 1 ng benzo[a]pyrene/m(3) represents a risk of DNA damage, as indicated by an increase in DNA adducts and the genomic frequency of translocations determined by FISH.
Related Concept Videos
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast, controlled...
Toxicity Testing in Animals
Pharmacokinetic–Pharmacodynamic Relationship: Exposure, Response and Effect
Biological Effects of Radiation
Criteria for Causality: Bradford Hill Criteria - II
Bioactivation and Tissue Toxicity
