Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Clinical Significance of Antibiotic Resistance

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

Mechanism of Antibiotic Resistance in MRSA

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Practice patterns in management of differentiated thyroid cancer since the 2014 British Thyroid Association (BTA) guidelines.

The surgeon : journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·2023

Same author

Complications and predisposing factors from a decade of total laryngectomy.

The Journal of laryngology and otology·2020

Same author

In Sickness and in Health: The Relationships Between Bacteria and Bile in the Human Gut.

Advances in applied microbiology·2016

Same author

Interpretation criteria in Western blot diagnosis of Lyme borreliosis.

British journal of biomedical science·2011

Same author

Is Tayside becoming a Scottish hotspot for Lyme borreliosis?

The journal of the Royal College of Physicians of Edinburgh·2011

Same author

Mitochondrial DNA background modifies the bioenergetics of NARP/MILS ATP6 mutant cells.

Human molecular genetics·2009

Search research articles

Related Experiment Video

Updated: May 29, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

A cost-effective protocol for screening patients for methicillin-resistant Staphylococcus aureus.

E M Dickson¹, M M Davidson, A J Hay

¹Department of Microbiology, Raigmore Hospital, Old Perth Road, Inverness IV2 3UJ, UK. elizabeth.dickson2@nhs.net

British Journal of Biomedical Science

|September 29, 2011

Summary

Rapidly identifying methicillin-resistant Staphylococcus aureus (MRSA) carriers is crucial. A modified latex agglutination test offers a faster, cheaper alternative to PCR for MRSA detection in clinical labs.

More Related Videos

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Related Experiment Videos

Last Updated: May 29, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

Area of Science:

Clinical Microbiology
Infectious Diseases
Diagnostic Development

Background:

Hospital-acquired infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a growing global concern.
Early identification of MRSA carriers is vital for preventing patient-to-patient transmission.
Current molecular methods for MRSA detection are often technically demanding and costly.

Purpose of the Study:

To evaluate a modified latex agglutination test (Mastalex-MRSA) for rapid MRSA identification.
To assess the test's performance on clinical samples from chromogenic agar plates.
To compare the cost-effectiveness and speed of the modified test against existing methods.

Main Methods:

Modification of the Mastalex-MRSA latex agglutination test for penicillin-binding protein 2' detection.
Testing on known MRSA strains and other organisms from chromogenic agar plates.
Analysis of 3050 patient samples, with presumptive positives evaluated using the modified kit.

Main Results:

The modified test identified MRSA on chromogenic agar plates, with some samples usable directly from overnight incubation.
A significant proportion of samples were suitable after a short incubation on blood agar.
The modified test costs Euro 15.15 per positive result, substantially less than PCR (Euro 35.00).
This protocol enables presumptive positive MRSA results approximately 24 hours earlier than current methods.

Conclusions:

The modified Mastalex-MRSA test provides a rapid, cost-effective method for presumptive MRSA identification.
This approach can significantly expedite the reporting of MRSA status in clinical laboratories.
The modified test offers a practical alternative to more complex molecular assays for early MRSA detection.