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Updated: May 29, 2026

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

Published on: July 5, 2024

Transplantation in zebrafish.

Pulin Li¹, Richard M White, Leonard I Zon

¹Harvard Medical School, Howard Hughes Medical Institute, Harvard Stem Cell Institute, Children's Hospital Boston, Boston, Massachusetts, USA.

Methods in Cell Biology

|September 29, 2011

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Zebrafish transplantation assays enable researchers to track labeled cells in vivo. This chapter details methods for embryo and adult zebrafish transplantation, crucial for developmental studies and stem cell research.

Area of Science:

Developmental Biology
Cell Biology
Transplantation Science

Background:

Tissue and cell transplantation are vital for studying cell potential, cell-environment interactions, and stem cells.
Distinguishing and tracing transplanted cells is essential for transplantation assays.
Zebrafish are an excellent model organism for transplantation due to their transparency and advanced imaging capabilities.

Purpose of the Study:

To discuss the rationale behind various zebrafish transplantation techniques in embryos and adults.
To provide detailed methods for key zebrafish transplantation procedures.

Main Methods:

Utilizing transgenic techniques for fluorescence labeling of donor cells.
Employing advanced imaging technologies for in vivo observation.

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Transplantation of Cells Directly into the Kidney of Adult Zebrafish

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Last Updated: May 29, 2026

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

High-resolution Cell Transplantation in Embryonic and Larval Zebrafish

Published on: July 5, 2024

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

Transplantation of Cells Directly into the Kidney of Adult Zebrafish

Published on: May 18, 2011

A Simple and Effective Transplantation Device for Zebrafish Embryos

A Simple and Effective Transplantation Device for Zebrafish Embryos

Published on: August 2, 2021

Focusing on blastula/gastrula transplantation, stem cell transplantation, and tumor transplantation in zebrafish.

Main Results:

Fluorescence-labeled donor cells can be effectively identified and traced in vivo in zebrafish.
Zebrafish embryos and adults provide a suitable platform for various transplantation assays.
Detailed methodologies are presented for specific transplantation types.

Conclusions:

Zebrafish transplantation is a powerful technique for in vivo cell tracing and developmental studies.
The chapter provides a comprehensive guide to essential zebrafish transplantation methods.
This approach facilitates research in developmental potential, cell interactions, stem cells, and tumor biology.