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Protein trafficking rates assessed by quantum dot quenching with bromocresol green

Cathleen D Valentine1, A S Verkman, Peter M Haggie

  • 1Department of Medicine, University of California-San Francisco, CA 94143, USA.

Traffic (Copenhagen, Denmark)
|September 29, 2011
PubMed

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Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...