Related Experiment Video
Updated: May 29, 2026

Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
Published on: February 16, 2015
L-amino acid oxidase-induced apoptosis in filamentous Botrytis cinerea
Chi-Hua Cheng1, Chia-Ann Yang, Shu-Ying Liu
1Institute of Biotechnology, National Dong-Hwa University, Hualien 97401, Taiwan, ROC.
Abstract:
As opposed to single-cell yeast and mammalian cell lines, apoptosis has not been greatly investigated in filamentous fungi because antibodies to the relevant fungal apoptosis-related proteins are not available commercially and because multicellular organisms cannot be studied using flow cytometry. Here we demonstrate how antibodies from a nonfungal source could be used to investigate this pathway. We show that apoptosis in the filamentous fungus Botrytis cinerea is triggered by the mitochondria-mediated caspase pathway, with release of the apoptotic factors cytochrome c, caspase 3, and caspase 9, on treatment with Trichoderma harzianum-derived L-amino acid oxidase.
Related Concept Videos
The Intrinsic Apoptotic Pathway
Antifungal Agents
Apoptosis
Autophagic Cell Death
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...
Caspases
The Extrinsic Apoptotic Pathway

