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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...

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Related Experiment Video

Updated: May 29, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Digital imaging mass spectrometry.

Casimir Bamberger1, Uwe Renz, Andreas Bamberger

  • 1Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037, USA. cbamberg@scripps.edu

Journal of the American Society for Mass Spectrometry
|September 29, 2011
PubMed
Summary

This study introduces a novel digital imaging mass spectrometer for rapid molecular visualization. The device offers true image acquisition, enabling faster analysis of biomolecular samples with improved image reconstruction.

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Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
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Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

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Last Updated: May 29, 2026

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry
06:21

Imaging of Biological Tissues by Desorption Electrospray Ionization Mass Spectrometry

Published on: July 12, 2013

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
09:38

Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Published on: November 26, 2013

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Surface Science

Background:

  • Mass spectrometric imaging (MSI) is crucial for visualizing molecular distributions in various fields.
  • Current MSI techniques are often slow due to spot-by-spot data acquisition and complex image reconstruction.
  • There is a need for faster and more direct imaging methods in MSI.

Purpose of the Study:

  • To develop and present a novel digital imaging mass spectrometer.
  • To demonstrate true imaging capabilities for time-of-flight mass separation.
  • To enable faster and more straightforward molecular distribution analysis.

Main Methods:

  • Utilized an imaging mass spectrometer with an ASIC Timepix array detector.
  • Employed matrix-assisted laser desorption/ionization (MALDI) for direct sample ionization.
  • Integrated ion optical means for time-of-flight mass separation.

Main Results:

  • Achieved spatial resolving power of (84 ± 35) μm and mass resolution of 45.
  • Capable of analyzing surface areas up to ~2 cm².
  • Demonstrated high hit-multiplicity, straightforward image reconstruction, and potential for high-speed readout (>4 kHz).

Conclusions:

  • The developed digital imaging mass spectrometer provides true image acquisition, akin to a digital camera.
  • This technology offers significant advantages in speed and image reconstruction simplicity.
  • Potential for rapid analysis of biomolecular samples in the near future.