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Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Infrared laser ablation sample transfer for MALDI and electrospray.

Sung-Gun Park1, Kermit King Murray

  • 1Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA.

Journal of the American Society for Mass Spectrometry
|September 29, 2011
PubMed
Summary

This study introduces infrared laser ablation to transfer samples into solvent droplets for mass spectrometry analysis. This method enables direct analysis of biological fluids like blood and milk.

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An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Published on: July 29, 2018

Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Biochemistry

Background:

  • Traditional sample preparation for mass spectrometry can be time-consuming and may introduce contaminants.
  • Ambient mass spectrometry techniques require efficient and direct sample introduction methods.

Purpose of the Study:

  • To develop and evaluate a novel infrared laser ablation method for ambient sample transfer into solvent droplets for mass spectrometry.
  • To assess the efficiency and applicability of this technique for analyzing various biological samples.

Main Methods:

  • Utilized a 2.94 μm infrared optical parametric oscillator (OPO) laser system for material ablation at atmospheric pressure.
  • Captured ablated material in solvent droplets suspended from a silica capillary for subsequent analysis.
  • Analyzed samples using matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry and nanoelectrospray ionization (nESI) mass spectrometry.

Main Results:

  • Successfully transferred picomole quantities of material to droplets with approximately 1% efficiency.
  • Demonstrated direct analysis of biological fluids including blood, milk, and egg.
  • Optimized parameters such as laser pulse energy, droplet size, and solvent composition were investigated.

Conclusions:

  • Infrared laser ablation offers a direct and efficient method for ambient sample transfer for mass spectrometry.
  • The technique is suitable for analyzing complex biological fluids, paving the way for ambient imaging applications.
  • This approach simplifies sample preparation and enhances the direct analysis capabilities of mass spectrometry.