Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Base Pairing02:27

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
DNA Base Pairing02:27

DNA Base Pairing

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
The DNA Helix01:16

The DNA Helix

Overview
The DNA Helix01:07

The DNA Helix

Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chalcogen substitution co-tunes photochromism and hydrogen bonding in semicarbazone photoswitches.

Chemical science·2026
Same author

Surface curvature effects in neutral and protonated water clusters: insights from DFT and energy decomposition analysis.

Physical chemistry chemical physics : PCCP·2026
Same author

How fluorine substituents strengthen aryl C-H bonds.

Chemical science·2026
Same author

Unlocking Hexahydrotriazine Chemistry for Multifold Thermoset Circularity.

Journal of the American Chemical Society·2026
Same author

The Nature of Nonclassical Chalcogeno-Carbonyl Ligands.

Inorganic chemistry·2026
Same author

Radical Stability Paradox: Substituent Effects versus Heats of Formation.

Chemistry (Weinheim an der Bergstrasse, Germany)·2025
Same journal

Ca<sup>2+</sup> effects on manganese oxidation by the bacterial multicopper oxidase complex Mnx.

Journal of inorganic biochemistry·2026
Same journal

The chemistry of the cobalt corrinoids - Recent advances and emerging themes. Part 3. Cobalamins and health.

Journal of inorganic biochemistry·2026
Same journal

PIKfyve-specific Pt(II)-based targeted drug conjugate in treatment of ovarian cancer through multi-mode actions.

Journal of inorganic biochemistry·2026
Same journal

From PET to targeted radionuclide therapy in the Brain: The emerging role of radiometal-based platforms.

Journal of inorganic biochemistry·2026
Same journal

The chemistry of the cobalt corrinoids - Recent advances and emerging themes. Part 2. The biochemistry, microbiology, and ecology.

Journal of inorganic biochemistry·2026
Same journal

Substituent effects in picolinic acid-derived silver(I) and zinc(II) complexes: Structure, stability, DNA interactions and therapeutic potential.

Journal of inorganic biochemistry·2026
See all related articles

Related Experiment Video

Updated: May 29, 2026

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

Silver(I)-mediated Hoogsteen-type base pairs.

Dominik A Megger1, Célia Fonseca Guerra, F Matthias Bickelhaupt

  • 1University of Muenster, Institute for Inorganic and Analytical Chemistry, Corrensstr. 28/30, 48149 Muenster, Germany.

Journal of Inorganic Biochemistry
|September 30, 2011
PubMed
Summary
This summary is machine-generated.

Researchers explored artificial nucleobases for metal-mediated Hoogsteen base pairs in DNA. Computational methods identified 1,3-dideaza-6-methoxypurine as a promising candidate for DNA construction.

More Related Videos

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Related Experiment Videos

Last Updated: May 29, 2026

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
09:04

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids

Published on: September 21, 2017

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Area of Science:

  • Biophysical Chemistry
  • Synthetic Biology
  • Computational Chemistry

Background:

  • Metal-mediated Hoogsteen-type base pairs are crucial for creating DNA duplexes with aligned metal ions.
  • Developing artificial nucleobases is key to controlling base pair stability and metal ion selectivity.

Purpose of the Study:

  • To theoretically evaluate artificial nucleobases for silver(I)-mediated Hoogsteen-type base pairs.
  • To understand how substituents and nitrogen atom presence affect base pair stability and bonding.

Main Methods:

  • Utilized dispersion-corrected density functional methods for theoretical evaluation.
  • Focused on silver(I)-mediated base pairs involving modified purines and cytosine.
  • Analyzed hydrogen and coordinative bond strengths, and overall base pair stability.

Main Results:

  • Investigated silver(I)-mediated Hoogsteen and reverse Hoogsteen base pairs with modified purines and cytosine.
  • Assessed the influence of C6 substituents and N3 atom absence on stability.
  • Found all artificial pairs less stable than the C-Ag(+)-G benchmark.

Conclusions:

  • 1,3-dideaza-6-methoxypurine forms a stable base pair with cytosine, comparable to the C-Ag(+)-C pair.
  • This modified nucleobase is a strong candidate for applications in DNA construction and engineering.